Abcam
Anti-Kallistatin / PI-4 antibody
ab187656
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My research focus is on understanding the role of the immune system in regulating blood glucose and adipose tissue. For this experiment, mouse liver was homogenized in RIPA lysis buffer and ran on a gel. Western blotting was performed to interrogate kallistatin expression.
Western Blot
Mouse liver
4% BSA in TBST overnight at 4°C
5% milk in TBST
5% milk in TBST for 2 hours at room temperature
N/A
ECL
Mouse liver was homogenized in RIPA lysis buffer and ran on a gel. Western blotting was performed to interrogate kallistatin for expression.
Good signal detection
Expensive antibody
This antibody worked well and detected a nice clear band. However Abcam antibodies are expensive.