Great Control Antibody for Phospho-AKT Signaling (AKT1)

March 19, 2019

Oncology
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Akt1 (C73H10) Rabbit mAb

Catalog Number:

2938

My research focus is on understanding the role of the immune system in regulating blood glucose and adipose tissue. For this experiment, HEK293T cells were serum starved and stimulated with insulin for different duration's to interrogate AKT signaling. AKT1 was used as a control for total AKT1 levels.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T cells

Primary Incubation

4% BSA in TBST overnight at 4°C

Blocking Agent

5% milk in TBST

Secondary Incubation

5% milk in TBST for 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ECL

Results Summary

HEK293T cells were serum starved and stimulated with insulin for different duration's to interrogate Phospho-AKT signaling. AKT1 was used a control for the total AKT1 protein.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great signal detection

The Bad

Expensive antibody

The Bottom Line

Great antibody for the detection of AKT1. It is needed control when looking at P-AKT signaling.

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