Good Control for P-S6 Signaling

March 13, 2019

Oncology
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

S6 Ribosomal Protein (5G10) Rabbit mAb

Catalog Number:

2217

My research focus is on understanding the role of the immune system in regulating blood glucose and adipose tissue. For this experiment, mouse CD4+ T-cells were stimulated with rapamycin. Total S6 was used as a loading control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

CD4+ mouse T-cells

Primary Incubation

4% BSA in TBST overnight at 4°C

Blocking Agent

5% milk in TBST

Secondary Incubation

5% milk in TBST for 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Mouse CD4+ T-cells were non-stimulated (left 4), stimulated (middle 4) and stimulated with rapamycin (right 4). Total S6 was used as a loading control.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great signal detection

The Bad

Expensive antibody

The Bottom Line

This antibody is a crucial control for anyone looking at P-S6 signaling. It shows a nice clear band.

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