Easy to Use with Fast Results

Duke University
Pharmacology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human XL Cytokine Kit

Catalog Number:

ARY022B

Our lab is interested in studying the role of inflammatory kinases as a therapeutic target. We perform Elisa and western blot assays to determine the role and pro inflammatory status of macrophages in response to pharmacological Inhibition of these kinases. This product is an easy-to-use screen for cytokines and chemokines. However, the analysis is time consuming as each dot needs to be quantified individually.

Experimental Design and Results Summary

Application

Protein Array

Starting Material

Supernatant

Protocol Overview

This is easy to sample extracellular proteins from immune cells following activation.

Tips

Optimize the dilution of protein for optimal detection.

Results Summary

Works well but needs to be optimized slightly and analysis is time consuming.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Fast and good screen.

The Bad

Analysis

The Bottom Line

Easy and works well.

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