New England Biolabs
α2-3,6,8 Neuraminidase
P0720S
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This enzyme was used to assess whether the SLeX moiety could be degraded to prevent selectin interaction with intact cancer cells. The enzyme worked moderately well over a two hour digestion, but results were messy.
Gkycobiology/Flow cytometry
Fut5 modified cancer cells
Cells were lifted from plates and digested with enzyme at 1:1000 for 2 hours at 37°C. They were then probed with recombinant Selectin reagents to assess the extent of SLex degradation by interaction measured via flow cytometry.
This may work better with free-floating proteins in a specialized solution, doesn't work great in PBS
Results showed that while the enzyme could cleave the sialic acid, it did not do so entirely and resulted in a messy signal.
N/A
It is the only enzyme that performs this reaction
Not a high turnover enzyme or may have other biophysical limitations
Works moderately, but not great for sensitive life science applications.