Santa Cruz Biotechnology
p53 antibody
SC-126
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Currently, there are no targeted therapies for triple negative breast cancer patients. Our lab focused on identifying novel molecules to develop targeted therapies for triple negative breast cancer patients. Our research program is involved in characterizing protein kinases and transcription factors as therapeutic targets for these patients. We used this antibody to detect p53 in western blot analysis.
Western Blot
Protein sample from human cancer cells
Antibody was diluted at 1:5000 in 5% milk for overnight at 4°C on shaker
5% milk in 0.01% TBS-T
Anti-mouse IgG conjugated with HRP diluted at 1:5000 for 1 hour at room temperature on shaker
N/A
ECL and Film
This antibody was used to detect wild-type p53 using western blotting technique.
Best antibody
Nothing
This is a great antibody to detect p53 and highly recommend this antibody.