Extremely Low Variability and Simple Running Platform

March 01, 2019

Princeton
Molecular Biology
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

QuantStudio 3 Real-Time PCR System

Catalog Number:

A28136

This system comes with a laptop setup and performs qPCR with exceptionally low variability and no abort rates. Display is somewhat lacking and computer has trouble connecting to university wireless server. Machine itself performs admirably.

Experimental Design and Results Summary

Application

q-RT-PCR

Protocol Overview

Product is used exclusively for 2-step qPCR to detect high and low expression genes with the sybr system. Can be run with the machine itself or via laptop connection.

Tips

Only 3 replicates are needed given such low variability

Features Summary

Remote operation, touchscreen operation

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Small footprint, high-fidelity qPCR, easy to use software

The Bad

Associated laptop has connectivity problems

The Bottom Line

Replaced our old qPCR very effectively, runs with low noise and with much better results. Limit of detection improved as well over old AB systems.

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