Withaferin Inhibits LPS-Induced TNFalpha Production in Macrophages

Medical University of Graz
Division of Cardiology
Lab Technician

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Company:

Tocris Bioscience

Product Name:

Withaferin A

Catalog Number:

2816/1

Withaferin is reported to have cardio-protective and anti-cancer effects.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

RAW macrophages

Protocol Overview

RAW macrophages were stimulated with Withaferin (1 µM) alone or in the presence of LPS (200 ng/ml) for 8 h to follow TNFalpha mRNA expression using qPCR.

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Results Summary

Withaferin significantly inhibited LPS-induced TNFalpha production.

DOI or PMID #

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Additional Notes

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Summary

The Good

Works as expected

The Bad

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The Bottom Line

Withaferin possesses a potent anti-inflammatory effect.

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