Staining Cultured MSC with Mouse IgG2b PE Isotype Control

January 30, 2019

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Mouse IgG2b PE isotype control

Catalog Number:

400314

This antibody was used as an isotype control to account for non-specific background staining in cultured MSC. It was chosen because it was the recommended isotype control for an antibody in a phenotyping panel, and Biolegend had a competitive price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human MSC

Primary Incubation

5 µL per test in 100 µL total staining volume for 15 minutes at room temperature.

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

Isotype staining was minimal (filled histogram) as compared to unstained cells (dotted line).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well. Reproducible results. Low price.

The Bad

N/A

The Bottom Line

Will continue to use this isotype control.

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