AMG 853 Blocks p38 Phosphorylation in 15d-PGJ2 Treated Cardiomyocytes

Medical University of Graz
Department of Cardiology
Postdoctoral Researcher

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Company:

Tocris Bioscience

Product Name:

AMG 853

Catalog Number:

5701/10

HL-1 cardiomyocytes were pre-incubated with AMG 853 (1 µM, 30 min) followed by treatment with 15d-PGJ2 (15 µM, 30 min) to follow p38 activation using western blot analysis. AMG 853 inhibited 15d-induced p38 phosphorylation.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

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Protocol Overview

HL-1 cardiomyocytes were pre-incubated with AMG 853 (1 µM, 30 min) followed by treatment with 15d-PGJ2 (15 µM, 30 min) to follow p38 activation using western blot analysis.

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Results Summary

AMG 853 inhibited 15d-induced p38 phosphorylation.

DOI or PMID #

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Additional Notes

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Summary

The Good

Works as expected

The Bad

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The Bottom Line

AMG 853 blocks p38 phosphorylation in 15d-PGJ2 treated cardiomyocytes.

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