Tocris Bioscience
Tempol
3082/100
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HL-1 cardiomyocytes were incubated with Tempol (1 mM) for 30 min prior to 15d-PGJ2 treatments (15 μM) for 1 hr to follow ROS generation using carboxy-H2DCFDA (10 μM). Figure shows that the antioxidant Tempol (a cell-permeable superoxide anion scavenger) blunted 15d-PGJ2-induced ROS formation efficiently.
Cell culture
N/A
HL-1 cardiomyocytes were incubated with Tempol (1 mM) for 30 min prior to 15d-PGJ2 treatments (15 μM) for 1 hr to follow ROS generation using carboxy-H2DCFDA (10 μM).
Figure shows that the antioxidant Tempol (a cell-permeable superoxide anion scavenger) blunted 15d-PGJ2-induced ROS formation efficiently.
Works as expected
Tempol blunts 15d-PGJ2-induced ROS levels in HL-1 cardiomyocytes.