Novus Biologicals
Fibronectin antibody
NBP1-91258
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This antibody was used in a study that investigated the cellular and molecular pathology in glaucoma. The antibody reviewed here gave great results in immunofluorescence staining of fibronectin on the trabecular meshwork.
Immunofluorescence
Pig eye fixed tissue section
1:400 for overnight
Normal donkey serum
Alexa fluor 488 conjugated donkey anti-rabbit antibody
Not applicable
AF 488 channel with epifluorescence microscope
This antibody worked excellently in immunofluorescence on tissue sections, shown as target-green and DAPI-blue. Only minimal background staining comparing to isotype control could be detected.
N/A
Great specificity
No
This is a very good antibody to detect fibronectin on tissue section. The minimal background staining is observed when compared to negative control.