Attune is Easy to Learn and Master

University of North Carolina at Chapel Hill
Medicine
Research Specialist

Overall

Performance

Ease-of-Optimization

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Company:

Invitrogen Thermo Scientific

Product Name:

Attune NxT Flow Cytometer with Attune NxT Autosampler

Catalog Number:

A24859

I am so grateful for the Attune NxT flow cytometer with the Attune NxT autosampler. I have not been doing flow cytometry for a very long time but I quickly was able to learn on this platform. It is a breeze to run 96 samples using the autosampler. I have been able to get so much data so easily. I just do my compensation, load my plate and then relax and poke at my gating. This is a dream come true for my research and my stress level. I have never had an issue with clogs. I have never had an issue with the autosampler. Flow rates are easy to optimize as are the voltages. I really do love this machine.

Experimental Design and Results Summary

Application

Flow Cytometry

Protocol Overview

200ul of cells at a density of 5x10^6 cells/ mL are loaded into 96 well round bottom plate in FACS buffer with single stains for compensation. Flow rate is 100ul/min. Voltages are adjusted based on single stains.

Tips

Do your single stains in tubes. Do your huge experiment in 96 well plate.

Features Summary

Autosampler, Flow Cytometer, Software

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to learn

The Bad

None yet

The Bottom Line

Easy to learn flow cytometry on this machine. Easy to run many samples.

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