R&D Systems
Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187)
MAB1018
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We were optimizing fixation and permeabilization conditions for primary T cells, and used pERK accumulation downstream of TCR stimulation as a test study. The R&D Systems pERK antibody worked fantastic!
Flow Cytometry
Jurkat
cRPMI
None
Anti-IgG Secondary
Attune Cytometer
Increased pERK with 10' (green) and 30' (blue) TCR stimulation with PMA/Iono
N/A
Very easy to use.
Nothing!
Great go-to pERK antibody for robust detection of phosphorylation downstream of TCR activation!