Cell Signaling Technology
Phospho-GSK-3β (Ser9) Antibody
9336
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The aim was to examine the levels of phospho-GSK-3ß C2C12 cells. Cells were transfected, harvested and equal amount of protein electrophoresed and transferred to nitrocellulose membrane followed by anti-phospho-GSK-3ß from CST. This antibody is easy to optimize and gives a single specific band.
Western Blot
C2C12
anti-pGSK-3b (1:1000 dilution), Overnight at 4 degree
Odyssey blocking buffer (1:1) ratio with washing buffer (PBST)
Goat anti-Rabbit IgG, LICOR (1:5000 dilution) at RT for 1hr
N/A
Scanned on Odyssey® CLx (auto scan 700 & 800).
I observed a single specific band corresponding at 46kD.
Very good
None
This p-GSK3Beta antibody from cell signaling gives beautiful band in western blots. This antibody also good for a wide range of cell origin such as human, mice, rat and monkey. This is also good for IHC and IF.