Cell Signaling Technology
Phospho-S6 Ribosomal Protein (Ser235/236)
#4858
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S6RP phosphorylation occurs downstream of mTOR signaling and is important for downstream signaling and activation of mRNA translation machinery.
Western Blot
Murine T Cell lysates
1/1000 4*C overnight in 5% BSA
5% BSA in TBS
1/1000 anti rabbit HRP, 1 hour room temp
Not required
Chemiluminescence
Clean strong band at expected mw
N/A
Reliable
Recommend for detection of S6RP activation