Invitrogen
FOXP3-APC monoclonal antibody for flow cytometry
17-5773-80
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Regulatory T cells are a specialized subset of CD4+ T cells involved in suppression of immune responses and identified by the expression of CD4+CD25+FOXP3+ markers. To detect the percent of regulatory T cells present in splenocytes after vaccine exposure, we have used this antibody in our antibody cocktail for multi-color flow cytometry.
Flow Cytometry
Mice splenocytes
1:20 dilution in perm buffer, 4°C, 1 hour (1–2 million cells)
Fc-block and fixation of cells for 30 minutes using intracellular fixation & permeabilization bufffer from eBioscience
None
Flow cytometry
Live CD4+ T cells were gated for the expression of CD25 and FOXP3 on dot plot. Double positive population (CD25+FOXP3+) is detected as regulatory T cells. Clear cloud for FOXP3+ cells were observed.
N/A
Always use the antibody with intracellular fixation & permeabilization buffer.
Good antibody for red laser detection by flow cytometry
For better results always use the antibody with eBioscience intracellular fixation & permeabilization buffer.