Good and Useful Kit for Plasmid Extraction for Transfection

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Quality of Results

Ease-of-Optimization

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Company:

Promega

Product Name:

Wizard® Plus SV Minipreps DNA Purification Systems

Catalog Number:

A1330

The transformed bacteria is grown and subsequently collected for plasmid extraction using this kit. The eluted plasmid is then used for transient transfection of Lentiviral production. The kit is easy to use and straightforward.

Experimental Design and Results Summary

Application

Plasmid Extraction

Starting Material

E. coli with plasmid

Protocol Overview

(1) Selected bacteria are grown and the pellet is collected. (2) Plasmids from the bacteria are eluted using manufacturer's protocol. (3) Collected plasmid is for further use.

Tips

Advised to use the endotoxin neutralizer in the kit if plasmid is intended for cell use.

Results Summary

A simple and straightforward kit to use. User-friendly kit!

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use and straightforward.

The Bad

Nil

The Bottom Line

Highly recommended kit for those who are isolating the plasmid for cell use.

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