Excellent Kit for In Vivo Analysis

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Overall

Quality of Results

Ease-of-Optimization

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Company:

Promega

Product Name:

VivoGlo™ Luciferin, In Vivo Grade

Catalog Number:

P1041

Cancer cells are transfected with the plasmid that encodes for the enzyme luciferase. After introducing the cells into the mouse models, the reagent is introduced into the mice and then they are further imaged to see the distribution and size of the cancer cells in the mice. The product is easy to use and works well.

Experimental Design and Results Summary

Application

IVIS imaging

Starting Material

AGS gastric cancer cell line

Protocol Overview

(1) Transfect the cell with the luciferase reporter. (2) Select the cells with antibiotics. (3) Inject cells into the mice. (4) Inject the diluted luciferin substrate. (5) Carry out IVIS imaging.

Tips

Ensure cells are successfully transfected with the luciferase reporter gene.

Results Summary

The results produced are accurate and sensitive. It is easy to administer the reagent without much hassle.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use and produce satisfactory results.

The Bad

NIL

The Bottom Line

A good reagent for in vivo imaging analysis.

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