High Fidelity DNA Polymerase Compared to the Conventional Taq Polymerase

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Quality of Results

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Company:

SmoBio

Product Name:

SMO-HiFi™ DNA Polymerase

Catalog Number:

TF1000

This reagent is commonly used for PCR to amplify our target gene of interest and used for subsequently cloning into our vector plasmid. It has a good fidelity rate.

Experimental Design and Results Summary

Application

PCR

Starting Material

DNA

Protocol Overview

Mix the recommended PCR mixture as stated in the manufacturer manual. Add the primers and template into the mixture. Run PCR to amplify target sequence.

Tips

DMSO can be substituted with water.

Results Summary

Sequencing results showed a good fidelity rate compared to conventional polymerases. Saves time for the users.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Faster reaction rate compared to other polymerases.

The Bad

NIL

The Bottom Line

High reaction rate (up to 1 kb/10 second). High fidelity, 70 times higher than Taq DNA polymerase. Thermo-stable-half life is more than 10 hrs at 95°C Highly recommended!

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