Reliable Nucleic Acid Quantification

Birla Institute of Technology Mesra, Ranchi, Jharkhand
Department of Bio-Engineering
Ph.D. Student

Overall

Performance

Ease-of-Optimization

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Company:

Promega

Product Name:

Quantus Fluorometer

Catalog Number:

E6150

In our lab, we regularly need an accurate reading of DNA/RNA concentrations for downstream molecular methods. While we employ additional quantitative methods, we needed an instrument that is accurate at low ranges. This unit is reliable at the low ranges it is advertised for.

Experimental Design and Results Summary

Application

Nucleic acid quantification

Protocol Overview

Generate a blank for your sample and read it in the mode determined by sample type. The reader makes use of 500 µL PCR microtubes. Use the user-friendly arrow keys and display to select the type of nucleic acid you want to quantitate. Read the sample.

Tips

Ensure you are clear on the dye to use based on your application. Become familiar with the manual.

Features Summary

We seem to achieve accurate readings at low concentrations.

DOI or PMID #

N/A

Additional Notes

History of readings is stored in the unit.

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Summary

The Good

User friendly and Accurate readings at low concentrations

The Bad

A little more prep time required than dye-less methods

The Bottom Line

Fairly cost effective, easy to use quantification at low concentrations.

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