Awesome Flow Cytometry Analyzer (12 Colors)

Johns Hopkins School of Medicine
Oncology
Postdoctoral Fellow

Overall

Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD FACSCelesta

Catalog Number:

BD FACSCelesta

As an immunologist, we heavily rely on flow cytometry for most experiments. Having the BD Celesta in our lab has greatly facilitated our research to perform multi-color flow cytometry. Routine experiments can easily be done by analyzing 12 colors. Using the Blue, Red, Violet (BVR) configuration we are able to cover most common flourophores. I recommend this product.

Experimental Design and Results Summary

Application

Flow Cytometry

Protocol Overview

Perform flow cytometry staining on single-cell suspension of samples. Obtain single stain compensation controls for auto compensation using BD FACS Diva software. Record controls to obtain compensation matrix to begin acquisition of samples depending on the colors.

Tips

Obtain good bright single stain controls for good matrix.

Features Summary

Easy-to-use software makes running samples easy especially if experienced user on DIVA.

DOI or PMID #

N/A

Additional Notes

Always best to understand the basic premises of flow cytometry.

Image Gallery

Summary

The Good

Small footprint. Fludicis are very quiet.

The Bad

None.

The Bottom Line

Amazing flow cytometer for immunologists interested in multi-color flow cytometry using 12 colors. Highly recommend!

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