MilliporeSigma/Merck
Anti-acetyl-Histone H3 (Lys9) Antibody
07-352
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Browse Similar Products: Acetyl-Histone H3 Western Blot Antibodies
Our aim was to examine the levels of H3K9 acetylation in HeLa cells treated with an inhibitor of cell growth. HeLa cells were treated with an inhibitor for 2 hours and whole-cell lysates were prepared by using NETN buffer. Samples were loaded on 15% SDS-PAGE and performed western blot. Levels of H3K9 acetylation were successfully analysed using this antibody.
Western Blot
HeLa whole-cell lysate
1:2000, Overnight at 4°C
5% skimmed milk, 1 hr at room temperature
1:10000, anti-rabbit HRP conjugated antibody, 1 hr at room temperature
N/A
ECL reagent, Chemi doc
We observed a single specific band corresponding to H3K9 acetylation in both control and inhibitor-treated cells at 17 kD on western blot.
29143563
Excellent antibody for performing western blots
A specific antibody which gives a specific band on western blot and easy to optimize