Cell Signaling Technology
Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody
9464
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Our lab studies mTORC2 signaling in macrophage polarization and function using Rictor KO macrophages. FoxO1 is downstream of this pathway since it acts as a transcription factor. Phosphorylation of FoxO1 leads to translocation from the nucleus to the cytoplasm. This antibody will detect the critical residues that leads to movement from the nucleus to the cytoplasm. This is a reliable antibody that is widely used in the scientific literature.
Western Blot
Murine macrophages
1:2000 in 4% BSA/TBST
5% dry milk/TBST
1:5000 Rabbit IgG HRP in 5% milk/TBST
None
Invitrogen ECL Pico
We are able to detect differential p-FoxO1/3 activity in our experimental conditions. The signal is quite robust since exposure time was low but this is based on our conditions and samples.
28877476
N/A
Reliable antibody to detect pFoxo1.3 at a good dilution
None really
One of the few antibodies on the market to truly detect p-FoxO1/3 activity. Widely used in the scientific community.