Cell Signaling Technology
FoxO1 (C29H4) Rabbit mAb
2880
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Browse Similar Products: FoxO1 Western Blot Antibodies
We are interested in studying FoxO1 regulation in macrophages and T cells since this transcription factor (TF) protein is controlled by mTOR signaling. Since this TF controls gene regulation, its stability and presence is a key readout to study FoxO1. We assess FoxO1 levels by immunoblotting. The antibody is robust and is widely used by the scientific community.
Western Blot
Murine macrophages
1:2000 in 4% BSA/TBST
5% dry milk/TBST
1:5000 Rabbit IgG HRP in 5% dry milk/TBST
None
Invitrogen ECL Pico
We are easily able to detect total FoxO1 levels in our conditions since we do not detect it in the expected condition but it is present in the other condition.
28877476
Titrate accordingly since it's quite robust.
Robust and very good antibody to detect Foxo1
Great antibody to detect Foxo1 that is widely used by the scientific community.