Cell Signaling Technology
Axin2 (76G6) Rabbit mAb
2151
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The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying the molecular mechanism of oncogenic WNT signal transduction to identify potential targets for therapeutic intervention of colon cancer. The goal of this experiment is to determine good working antibodies for the purpose of blotting some of the core components of the WNT pathway. This is antibody picks up two bands and is excellent for western blotting.
Western Blot
SW480 (whole-cell extract)
1:1000 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% BSA in TBS-T
1:10,000 dilution of donkey anti-rabbit IgG+HRP; 1 hr at room temperature
None
ECL
This antibody picks up bands at ~95 and ~98 kDa
N/A
Detection of specific bands
Excellent antibody for western blotting