Invitrogen (ebioscience)
CD127 Monoclonal Antibody (A7R34), APC
17-1271-82
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Our lab studies CD8 T cell differentiation into effector and memory cells. During an acute response, surface expression of the IL-7 receptor (CD127) has been shown to identify early memory precursors that become long-lived memory cells. Using this marker and KLRG1, we are able to identify these memory-like cells.
Flow Cytometry
Murine Splenocytes from infected mouse
1:250
FC block (10ug/mL)
None
BD Celesta
We are able to identify a strong single positive population of cells expressing CD127. These cells are interesting since they have the most potential to become memory cells.
N/A
Stains for CD127 very well. Antibody is not too expensive since APC.
Higher dilution is needed.
Great robust antibody to stain for CD127 on murine T cells.