Gibco
Trypan Blue Solution, 0.4%
15250061
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We routinely manually count cells using a hemacytometer. Trypan blue helps us determine the cell concentration.
Cell culture counting
Cells in media
We mix our biological sample of cells with trypan blue to help count viable cells. We typically dilute the trypan blue to .04% in PBS since 0.4% seems too strong for our counting.
Dilute 1:10 in PBS to make it easier to count while saving reagent.
We are able to get appropriate cell concentration by counting viable cells (not blue) from non-viable (blue) using the hemacytometer.
N/A
Easy to use and inexpensive
None
This trypan blue is a quick, easy, and reliable way to be able to manually count your cells with a hemacytometer.