p63 Antibody Works on Mouse!

Pathology
UCSF
Graduate Student

Overall

Quality of Results

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Company:

Biolegend

Product Name:

anti-deltaNp63 antibody

Catalog Number:

619002

There are limited reagents for detecting Tp63 isoforms in mouse tissue. This human-specific antibody works perfectly well on mouse tissue as well!We stained mouse mammary glands with Biolegened 619002 anti-deltaNp63 as well as Genetex 102425 anti-p63 (which is known to recognize all isoforms of mouse p63).

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse Mammary Gland

Primary Incubation

1:500, 30 min at room temperature, in 10% goat serum + PBST

Blocking Agent

10% goat serum in PBST, 1 hour at room temperature

Secondary Incubation

Vectastain Elite anti-rabbit biotinylated secondary, prepared as according to manufacturers protocol, 30 min at room temperature

Tertiary Incubation

Vectastain Elite ABC reagent, prepared according to manufacturers protocol, 30 min at room temperature

Detection

NovaRed

Results Summary

This human-specific antibody appropriately labels the myoepithelial/basal populations of mouse mammary gland. The staining mirrored that of an established Total p63 antibody GTX 102425

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works on mouse tissue

The Bad

None

The Bottom Line

This antibody works on mouse tissue (although not described on manufacturers website).

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