Biolegend
PE/Cy7 anti-human CD186 (CXCR6) Antibody
356012
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This anti-CXCR6 (CD186) antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes (from human PBMCs). We are particularly interested in the innate compartment of lymphocytes. CXCR6 is a chemokine receptor, also known as CD186, is used as a marker for activated and memory T lymphocytes in this panel.
Flow Cytometry
Human PBMCs
1:50, 37°c for 15min
PBS/BSA
N/A
Flow cytometer
Antibody identified the CXCR6+ cells within PBMCs, however it is not exceedingly bright. No absolute separation of negative and positive populations was obtained at any titration.
Identified CXCR6pos cells
Resolution not the highest
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