Santa Cruz Biotechnology
TGN38 Antibody (B-6)
sc-166594
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My experiment was focused on evaluating the effect of a variant on the localisation of a secreted protein. My hypothesis was that the variant could impair the physiologic protein secretion. I used the anti-TGN38 antibody as a marker of Golgi apparatus, to localise the wild-type and mutant protein along the secretory pathway. This antibody was used for immunofluorescence on HEK293 cells fixed with paraformaldehyde 4%.
Immunofluorescence
HEK293 cell line
Antibody diluted 1:75 in blocking solution, incubated for 2 hrs at RT
BSA 2%
Dylight 549 goat anti-mouse secondary antibody (115-505-174) diluted 1:200 in blocking solution 1 hr at RT
N/A
Olympus FluoView FV1000
The antibody works in immunofluorescence on PFA 4% fixed cells, but some background remains.
Fine for immunofluorescence
Some background
The antibody works in immunofluorescence on paraformaldehyde fixed cells, but could perform better.