Cell Signaling Technology
Anti-NeuN Rabbit mAb
24307
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Browse Similar Products: NeuN Immunofluorescence Antibodies
This antibody gave us reproducible and high quality staining of neuronal nuclei in vibratome-sliced fixed mouse brains. This enabled us to localize microglia and neurons simultaneously and study their spatial relation.
Immunofluorescence
Vibratome-sliced fixed mouse brain
1:1000, overnight at 4degrees C
5% Normal Goat Serum and 5% BSA
1:1000 Gt anti-Rb Cy3, 1h at RT
N/A
Confocal laser scanning microscopy, 60x objective
This Ab was used with a red secondary Ab on brain slices from mice expressing GFP in microglia. This enabled us to localize microglia and neurons simultaneously and study their spatial relation. Since the Cy3 signal faded similar to gfp with deeper confocal scanning, the Ab successfully penetrated the entirety of our thick (100um) slice preparation.
Great neuronal nuclei marker
Doesn't get the full morphology of the neuron, obviously
A no-brainer, widely used monoclonal Ab for identifying neuronal nuclei