Promega
CytoTox-ONE™ Homogeneous Membrane Integrity Assay
G7891
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Our laboratory works on engineering T cells and stimulating NK cells for enhanced antiviral activity against HIV-infected cells. As such, we set up in vitro killing assays where it is crucial to identify dead target cells. This kit allows detection of LDH release by converting it into a fluorescent product.
Cytotoxicity Assay
HIV infected cells co-incubated with effector cells
Follow kit's manual
Make sure you optimize the time window for the assay. Depending on target cell lines extended incubations can lead to high background
In general kit works, however it is a bit dependent upon target cells used. When we use primary infected cells we get high backgrounds, when we use ACH2 cells (hard to kill) it is difficult to detect cells that are dying.
N/A
It works
Needs specific optimizations for specific target - effector pairs
It was a method to detect cell death during cytotoxicity assays