Good PCNA Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PCNA Antibody

Catalog Number:

307909

In this project the pathogensis of melanoma was investigated. With the MELC technology, we analyzed protein expression profiles in nevi and melanomas. This antibody was used to study the PCNA-expression in human melanoma cell lines by direct immunfluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human melanoma cell line

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (Alexa Fluor 488) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific and good signals for immunocytochemistry with some background. Publishable results!

DOI or PMID #

none

Additional Notes

none

Related Categories

Image Gallery

Summary

The Good

Specific signals, easy to use!

The Bad

Some background

The Bottom Line

Will buy and use it again. Recommendable!

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