Antibody Stains Actively Proliferating Cells Well

June 12, 2018

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Histone H3 (Ser10) Antibody

Catalog Number:

9701

We wanted to assess proliferation of cells in a healthy vs diseased state, so we stained for phospho-histone H3 to identify actively proliferating cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse large intestine

Primary Incubation

1:100 in 1% BSA in PBS overnight

Blocking Agent

5% goat serum in 1% BSA

Secondary Incubation

1:200 in PBS for 1 hour at room temperature

Tertiary Incubation

None

Detection

Confocal microscopy

Results Summary

Actively proliferating cells are stained well with the antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Stains phosphorylated histone 3 very well

The Bad

none to mention

The Bottom Line

The antibody is great for identifying actively dividing cells

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