Detecting Proteins Using Bradford Assay

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Performance

Ease-of-Optimization

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Company:

Molecular Devices

Product Name:

Spectramax2

Catalog Number:

M2

The aim of this experiment was to measure the amount of protein extracted using the Bradford protein assay. The Spectramax2 plate reader was used to detect the absorbance. This plate reader was selected as it was available in house and recommended by colleagues.

Experimental Design and Results Summary

Application

Measuring absorbance and fluorescence

Protocol Overview

Place the plate on the reader and follow the instructions on the software.

Tips

Turn on the machine, remove the lid from plate.

Features Summary

Simple to use.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Reliable.

The Bad

None

The Bottom Line

Very easy to operate, quick and reliable. No special expertise required.

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