Thermal Cycler For PCR And Real-Time Reactions

May 24, 2018

The Scripps Research Institute
The Cell and Molecular Biology
Assistant Professor

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

C1000 Thermal Cycler

Catalog Number:

185-1048

Thermal cyclers are used to run polymerase chain reaction (PCR), in particular RT-PCR, to determine the transcription level of a gene of interest. The C1000 Thermal Cyclers from Bio-Rad are compact instruments compatible with different 1000-series reaction modules. Our machine is equipped with a "Dual-48" module that has 2 independent 48-well blocks. It also has a touch screen, which is user-friendly and convenient.

Experimental Design and Results Summary

Application

PCR

Starting Material

RNA from Human tumor cells

Protocol Overview

Human tumor cells are harvested and RNA is immediately isolated from the cell pellet with TRIzol reagent. Three microgram of purified RNA are subjected to cDNA synthesis using Takara/Clontech "RNA to cDNA EcoDry" kit. The samples volumes are between 30 and 40 microliters. PCR protocol includes: initial denaturation and activation step (94oC for 3 min), followed by 35 cycles of: denaturation at 94oC 30 sec, annealing of primers at 55oC for 30 sec, synthesis of the complementary strand at 72oC for 1 min.

Tips

The cycler should have sufficient air flow to prevent overheating. The blocks should be cleaned regularly.

Features Summary

The C1000 cycler is a compact and reliable instrument, which is easy to use and program.

DOI or PMID #

N/A

Additional Notes

The built-in and saved protocols could be quickly optimized to fit the requirements of a particular protocol.

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Summary

The Good

The instrument is compact and can be placed on a standard-size bench. This cycler is easy to set up and use.

The Bad

If well-maintained and used as instructed, the C1000 cycler does not have any bad features.

The Bottom Line

The C1000 thermal cycler from Bio-Rad is a reliable and friendly instrument that could be recommended to any research lab in need of running PCR assays.

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