Time Course Effect Of Chemoattractant On Cleaved Caspase-3 Expression

April 17, 2018

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

Cleaved Caspase-3

Catalog Number:

9661

Caspase-3 is a member of cysteine aspartic acid protease family. CASP3 is akey regulator for apoptosis, and it is responsible for proteolytic cleavage of many proteins such as poly (ADP-ribose) polymerase (PARP1). Here we checked the time course effect of chemoattractant on CASP3 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% BSA prepared in 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

There is no significant change in CASP3 expression.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody works great. Highly recommended.

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