The Antibody Stains B220 Very Well

April 19, 2018

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103212

We wanted to look at B cell numbers and whether they changed in the disease vs healthy state, so we used this antibody to identify B cells in the colon.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse colon cells

Primary Incubation

1:100 in flow staining buffer

Blocking Agent

1:100 FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody stains B220+ cells very clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody stains B220+ cells clearly

The Bad

None to mention

The Bottom Line

The antibody is good for identifying B220+ cells

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