Great Smooth Muscle Actin Antibody

March 22, 2018

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate Student

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Company:

MilliporeSigma

Product Name:

Anti-Actin, α-Smooth Muscle antibody

Catalog Number:

A5228

Browse Similar Products: Actin Immunohistochemistry Antibodies

We compared extent of myofibroblast differentiation in samples between treated and untreated conditions. It is a widely used smooth muscle actin marker.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Pig Skin

Primary Incubation

1:200 at 4 degrees overnight

Blocking Agent

5% NGS

Secondary Incubation

1:500 biotinylated anti-mouse antibody for 1 hour at room temperature

Tertiary Incubation

AB kit

Detection

DAB

Results Summary

Clean identification of bands. Use Tris/EDTA pH9 buffer for hear mediated antigen retrieval.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works the well.

The Bad

Expensive

The Bottom Line

Effective antibody to identify SMA.

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