Santa Cruz Biotechnology
JAK1 Antibody (B-3)
sc-376996
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The canonical JAK-Stat pathway is a part of a normal immune system and can cause proliferation, differentiation, survival, and host resistance to pathogens. We examined the effect of an inhibitor on JAK-1 in Jurkat cells by Western blotting.
Western Blot
Cell Lysates of Jurkat
1:500 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:10000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
JAK-1 specific bands were observed in the western blotting.
N/A
Specificity and reactivity
Nothing
In total, this antibody works really well for western blotting.