Expression of CD127 on Human PBMC

March 19, 2018

Microbiology Immunology
University of Texas Medical Branch
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CD127 antibody

Catalog Number:

351325

The CD127 antibody tagged with brilliant violet 650 stains human PBMCs successfully. A wide array of antibodies were tested for surface expression of CD132 family of members.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30 minutes on ice

Blocking Agent

PBS with 5% BSA

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Antibody works excellently

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Antibody works excellent in staining cells

The Bad

None

The Bottom Line

Antibody works for staining CD127

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