Cell Applications, Inc.
Porcine Aortic Endothelial Cells: PAOEC
P304-05
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The general aim was to study the intercellular communication between trophoblasts and endothelial cells via extracellular vesicles (EVs; Exosomes). We demonstrated the stimulatory effects of EVs derived from porcine trophectoderm-cells on various biological processes including the proliferation of maternal endothelial cells (Porcine Aortic Endothelial Cells), potentially promoting angiogenesis during porcine pregnancy. Porcine Aortic Endothelial Cells are the ideal cells for specific aims in our study. To my knowledge, Cell Applications was the only provider of this cell line during our experimentation. I recommend this to anyone looking for endothelial cells to study angiogenesis.
Isolation of extracellular vesicles derived miRNAs and mRNAs
Porcine aortic endothelial cells (PAOEC) were purchased from the Cell Applications, Inc., San Diego, CA, USA. PAOEC’s were maintained according to manufacturer’s instructions using the porcine endothelial cell medium (P211-500) and subcultured using subculture reagent kit (090 K; Cell Applications, Inc., San Diego, CA, USA). Supernatant from PAOEC cultures was collected for exosome isolation.
As company suggests, you cant maintain this cell line for many passage (Not more than 7-8)
Exosomes and microvesicles are extracellular vesicles released from cells and can contain lipids, miRNAs and proteins that affect cells at distant sites. Recently, microvesicles containing miRNA have been implicated in the uterine microenvironment of pigs, a species with unique epitheliochorial (non-invasive) placentation. Here we report a novel role of conceptus-derived exosomes/microvesicles (hereafter referred to as extracellular vesicles; EVs) in embryo-endometrial cross-talk. We also demonstrate the stimulatory effects of EVs (PTr2-Exo) derived from porcine trophectoderm-cells on various biological processes including the proliferation of maternal endothelial cells (PAOEC), potentially promoting angiogenesis. Transmission immuno-electron microscopy confirmed the presence of EVs in tissue biopsies, PTr2-Exo and PAOEC-derived EVs (PAOEC-Exo). RT-PCR detected 14 select miRNAs in CD63 positive EVs in which miR-126-5P, miR-296-5P, miR-16, and miR-17-5P were the most abundant angiogenic miRNAs. Proteomic analysis revealed EV proteins that play a role in angiogenesis. In-vitro experiments, using two representative cell lines of maternal-fetal interface, demonstrated bidirectional EVs shuttling between PTr2 and PAOEC cells. Importantly, these studies support the idea that PTr2-Exo and PAOEC-Exo containing select miRNAs and proteins can be successfully delivered to recipient cells and that they may have a biological role in conceptus-endometrial cross-talk crucial for the pregnancy success.
10.1038/srep40476
N/A
Easy to grow and handle
Can't be maintained after 10 passages, they appear to loose their morphology.
Highly recommended