Anti-Mouse F4/80 Pacific Blue Antibody

October 23, 2017

Medical Oncology
DFCI
Postdoc

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Company:

Biolegend

Product Name:

F4/80 Pacific Blue

Catalog Number:

123124

My research aim is to investigate the response of the immune system after immune-therapy. The experiment was performed to show change in macrophages and microglia population in tumor-bearing mice. Pacific Blue anti-mouse F4-80 has been chosen because is a good clone/color.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor-infiltrating lymphocytes in GL261 tumor-bearing mice.

Primary Incubation

30 minutes

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa

Results Summary

After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with F4/80 1:100, wash 2x, fix in PFA2% or proceed with intracellular staining protocol

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Quality/price ratio

The Bad

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The Bottom Line

Good antibody to use alone or in combination with CD11b to identify macrophages ex vivo.

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