Anti-Mouse CD25 (APC) Antibody

October 04, 2017

Cell Biology
La Jolla Institute for Allergy and Immunology
Instructor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse CD25 Antibody

Catalog Number:

102012

The aim was to study T helper cell subsets. The specific goal was to examine CD25+ regulatory T cells from mouse CD4 T cells. I selected this product because it was of high quality and is cost effective.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 (0.4ul of antibody in 40ul of sample)

Blocking Agent

5% rat serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

APC

Results Summary

CD25 expression in Foxp3+ T cells was determined with this antibody

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

High quality

The Bad

None

The Bottom Line

This antibody working well with high sensitivity/specificity

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