Themo Fisher Scientific
LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit
L34957
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I am interested in characterizing different immune cell populations in the murine colonic lamina propria in the context of colon cancer. As a first step we utilized the Fixable Aqua Dead Cell Stain Kit to gate out dead cells.
Flow Cytometry
Mouse colon tissue sample
The reagent was used at 1:1000 dilution and the manufacturers instructions were followed
None
Dead cells are clearly LD Aqua positive compared to live cells
N/A
Staining is clear
The separation between positive and negative populations is sometimes not very clear
The kit is good enough