FOXP3 Monoclonal Antibody for Flow Cytometry

September 13, 2017

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

FOXP3 Monoclonal Antibody (FJK-16s), APC, eBioscience™

Catalog Number:

17-5773-82

I am interested in studying the homing of T cells from the peripheral lymph nodes to the murine colonic lamina propria in the context of colon cancer. Therefore, as a first step we optimized the stain for regulatory T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse peripheral lymph node sample

Primary Incubation

1:50 dilution; 20 min at room temperature using FOXP3 staining kit from eBioscience

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

FOXP3 positive population is clearly distinguished with this antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Staining is clear

The Bad

None

The Bottom Line

This product is good

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