Invitrogen Antibodies by Thermo Fisher Scientific
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647
A32733
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This secondary antibody was used to perform indirect immunofluorescence microscopy on HeLa cells to an HA-tagged protein. It showed strong immunoreactivity that was consistent the cytosol. Background levels were low. This antibody is a good choice for immunofluorescent microscopy. There is little to no difference between this secondary and the non plus version.
Immunofluorescence
HeLa cells fixed with 3.7% paraformaldehyde
1 hour at room temperature in blocking agent at 1:300
PBS in 1% normal goat serum and 0.1% triton x-100
1 hour at room temperature (1:300)
DAPI
Confocal Microscopy
This secondary should good results in HeLa cells. This version is more expensive than the non-plus but the results between the two were comparable.
N/A
works well
Expensive
Save some money and stick with the non-plus version