Cell Signaling Technology
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9)
4511
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p38 MAPK is one of the class of mitogen activated protein kinase which will activated by various stimuli like cytokines, UV, heat shock and other osmotic shocks and it is known to be involved in cell differentiation, apoptosis and autophagy. Here we checked the time course effect of p38 by chemoattractant.
Western Blot
Human lung cells
1:1000 dilution prepared in 1x TBST
5% BSA prepared in 1x TBST
1:4000 dilution prepared in 1x TBST
None
ECL
We observed time dependent increase of p38 phosphorylation in human lung cells.
N/A
This antibody is very good for western blot experiment
We have to use atleast 1:1000 dilution otherwise the signal is very weak.
This antibody is very specific for human and mouse samples.Highly recommended.