Review of CD73 PerCP-Efluor710

Research
Bloodworks NW
Postdoctoral research fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), PerCP-eFluor 710, eBioscience™

Catalog Number:

46-0731-82

Based on prior publications by other groups, we wanted to study CD73hi FR4hi T cells and this antibody was used in conjunction with FR4 PeCY7 to detect our population of interest.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30 mins @ +4C

Blocking Agent

Anti-CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

Splenocytes were stained with antibodies CD4, CD73 and FR4. Data shown is gated on CD4+ T cells. We observed a small population of cells that expressed high levels of CD73 and FR4.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great separation between positive and negative populations.

The Bad

None

The Bottom Line

Highly recommend.

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